HTS for sortase A inhibitors using MALDI MS and polymeric enrichment arrays (PEAs)

The rise of antibiotic-resistant Staphylococcus aureaus necessitates new therapeutics to combat infections. Sortase A is an essential enzyme for virulence and a promising therapeutic target. Mass spectrometry combined with polymeric enrichment arrays (PEAs) offers a label-free and high-throughput technology to measure the protease and ligase activities in a multiplexed readout. Discover how Peapod Bio utilized this innovative approach to screen 200,000 compounds in days to reveal novel sortase A inhibitors.